Journal: EMBO Molecular Medicine

Article Title: Integrated multi-omics mapping of mitochondrial dysfunction and substrate preference in Barth syndrome cardiac tissue

doi: 10.1038/s44321-025-00320-5

Figure Lengend Snippet: ( A ) Overview of cardiolipin biosynthesis and remodelling via the Tafazzin enzyme, which is dysfunctional in BTHS. ( B ) Relative intensity of CL(72:8), MLCL(50:2) and the ratio between MLCL(50:2) and CL(72:8) in both BTHS ( n = 5) and non-failing donors ( n = 24). Boxplots represent the interquartile range (IQR), with the box spanning the 25th to 75th percentiles and the median indicated by a horizontal line. Whiskers extend to the most extreme data points within 1.5×IQR. Statistical comparisons were performed using moderated t-tests from the limma package in R, with Benjamini–Hochberg correction. Annotated p -values are defined as follows: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ( C ) Volcano plot of CL species, depicting depleted CL species (blue) and accumulated CL species (red) in cardiac tissue of BTHS individuals ( n = 5) compared to non-failing donors ( n = 24). ( D ) Relative intensity of the total MLCL and DLCL classes in BTHS individuals ( n = 5) and non-failing donors ( n = 24). Boxplots are defined as in ( B ). Statistical comparisons were performed using moderated t-tests from the limma package in R, with Benjamini–Hochberg correction. Annotated p -values are defined as in ( B ). Exact p -values corresponding to all statistical comparisons are provided in: Dataset (STATISTICS LIPIDOMICS), Dataset (STATISTICS METABOLOMICS), and Dataset (STATISTICS PROTEOMICS).

Article Snippet: Cardiolipin CL(14:0)4 , Avanti Polar Lipids, Alabaster, AL, USA , 750332.

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